While the MSAFP test is a valuable screening tool, it has limitations:
1. [False Positives](https://): Elevated AFP levels can sometimes occur without any fetal abnormalities, leading to unnecessary anxiety and additional testing. 2. [False Negatives](https://): Normal AFP levels do not guarantee the absence of disorders, as some conditions may not affect AFP levels. 3. [Timing](https://): The accuracy of the test depends on the precise dating of the pregnancy. Incorrect gestational age can affect the interpretation of AFP levels. 4. [Not Definitive](https://): The MSAFP test is not diagnostic; it only indicates the need for further testing.
