dna quality dependency

How is DNA Quality Assessed?

DNA quality is typically assessed through several parameters, including its concentration, purity, and integrity. Methods such as spectrophotometry, gel electrophoresis, and fluorometry are commonly used. High-quality DNA should have an A260/A280 ratio between 1.8 and 2.0 and show minimal degradation in electrophoresis. These assessments ensure that the DNA is suitable for downstream applications like sequencing, PCR, and cloning.

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