DNA methylation analysis involves several techniques: - Bisulfite Sequencing: Converts unmethylated cytosines to uracil, allowing for the differentiation between methylated and unmethylated cytosines. - Methylation-Specific PCR (MSP): Amplifies DNA regions with specific methylation patterns. - Pyrosequencing: Provides quantitative data on methylation levels across multiple CpG sites. - Microarray-Based Methods: Allows for high-throughput analysis of methylation patterns across the genome.
