RISC functions by incorporating small RNA molecules, such as small interfering RNA (siRNA) or microRNA (miRNA), and using them as guides to identify complementary mRNA targets. Once the target mRNA is recognized, RISC either degrades the mRNA or inhibits its translation, effectively silencing the gene. This post-transcriptional regulation is vital for controlling gene expression during development and differentiation, processes that are especially critical in pediatric growth and development.