Children often present with non-specific symptoms that can be attributed to a variety of infectious agents. Traditional diagnostic methods can be time-consuming and may require multiple tests, which delays diagnosis and treatment. Multiplexing allows for: 1. Rapid diagnosis: Multiple pathogens can be identified in a single test, leading to faster treatment. 2. Comprehensive screening: Detects co-infections that may be missed with single-pathogen tests. 3. Reduced sample volume: Less invasive, requiring smaller sample sizes, which is particularly important for pediatric patients.