Cross-linking: Cells are treated with a cross-linking agent, such as formaldehyde, to fix proteins to DNA. Shearing: The DNA-protein complexes are then sheared into smaller fragments using sonication or enzymatic digestion. Immunoprecipitation: An antibody specific to the protein of interest is used to precipitate the DNA-protein complex. Purification: The DNA is purified from the protein-DNA complex. Analysis: The purified DNA is analyzed using techniques like PCR, qPCR, or sequencing to identify the specific DNA sequences bound by the protein.
