DNA extraction: Obtain DNA from pediatric samples such as blood, tissue, or saliva. Bisulfite treatment: Treat the DNA with sodium bisulfite to convert unmethylated cytosines to uracils. Primer design: Design primers specific for methylated and unmethylated DNA sequences. PCR amplification: Use the designed primers to amplify the target regions in the treated DNA. Analysis: Evaluate the PCR products through gel electrophoresis or other methods to determine the methylation status.
