DNA Extraction: DNA is extracted from the child's cells, typically from a blood sample. Amplification: The region of interest in the DNA is amplified using Polymerase Chain Reaction (PCR). Sequencing Reaction: The amplified DNA is subjected to the sequencing reaction, where dideoxynucleotides terminate the DNA synthesis at specific points. Electrophoresis: The resulting DNA fragments are separated by size using capillary electrophoresis. Data Analysis: The sequence data is analyzed to identify any mutations or variations.
