While chromosomal analysis is a powerful diagnostic tool, it has limitations. It may not detect very small chromosomal deletions or duplications, also known as microdeletions or microduplications. In such cases, more advanced techniques like fluorescence in situ hybridization (FISH) or comparative genomic hybridization (CGH) may be required. Additionally, chromosomal analysis cannot identify single-gene disorders or mutations that do not affect the structure or number of chromosomes.
