The process of single cell analysis typically involves several steps:
Cell Isolation: Techniques like microfluidics, fluorescence-activated cell sorting (FACS), and laser capture microdissection are used to isolate individual cells from a tissue sample. Library Preparation: Each cell's RNA or DNA is extracted and converted into a library of sequences that can be analyzed. Sequencing: High-throughput sequencing technologies, such as next-generation sequencing (NGS), are employed to read the genetic material from each cell. Data Analysis: Bioinformatics tools are used to analyze and interpret the vast amount of data generated, identifying patterns and differences among cells.
