Preparation: The neonate is positioned appropriately, and the area to be aspirated is cleaned and sterilized. Anesthesia: Local anesthesia may be administered to minimize discomfort. In some cases, sedation may be used. Insertion: A thin, sterile needle is carefully inserted into the targeted area under ultrasound guidance or other imaging techniques to ensure accuracy. Aspiration: Fluid, air, or tissue is withdrawn through the needle. The sample may be sent for further laboratory analysis. Post-Procedure Care: The site is monitored for any signs of complications, and the neonate is observed to ensure stability.