The process of multiplex PCR involves several steps:
Sample Collection: Samples such as blood, saliva, or tissue are collected from the patient. DNA/RNA Extraction: The genetic material is extracted from the collected sample. Primer Design: Specific primers for the target genes or pathogens are designed and used in the PCR reaction. Amplification: The PCR reaction is carried out in a thermocycler, where multiple targets are amplified simultaneously. Detection: The amplified products are then detected and analyzed using various techniques such as gel electrophoresis or real-time PCR.
