Several tests and procedures are used to diagnose Hirschsprung disease:
Rectal biopsy: This is the most definitive test where a small piece of the rectum is examined for the absence of ganglion cells. Contrast enema: This imaging test uses a special dye to highlight the colon and identify any abnormalities. Anorectal manometry: This test measures the muscle movements in the rectum and anus to detect abnormalities.
