The process of bisulfite sequencing involves several steps:
DNA Extraction: Isolate DNA from tissue or blood samples. Bisulfite Treatment: Treat the DNA with sodium bisulfite to convert unmethylated cytosines to uracil. PCR Amplification: Amplify the treated DNA using polymerase chain reaction (PCR). Sequencing: Sequence the amplified DNA to identify methylation patterns. Data Analysis: Analyze the sequencing data to determine the methylation status of specific CpG sites.
