The preparation of a wet mount involves several steps:
Sample Collection: A sample (e.g., vaginal discharge, stool, skin scraping) is collected using sterile techniques. Placement on Slide: The sample is placed on a glass slide. Adding Solution: A drop of saline or iodine solution is added to the sample to enhance visibility of the organisms. Covering: A coverslip is placed over the sample to flatten it and prevent drying. Examination: The slide is examined under a microscope at varying magnifications to identify any microorganisms.