NGS involves several key steps: 1. Sample Preparation: DNA or RNA is extracted from a sample, such as blood or tissue. 2. Library Preparation: The genetic material is fragmented, and specific adapters are attached to the ends. 3. Sequencing: The prepared library is loaded onto a sequencer, which reads the genetic material in fragments. 4. Data Analysis: Bioinformatics tools align the sequenced fragments to a reference genome, allowing for the identification of genetic variants.