Chromatin Preparation: Cells are treated to cross-link proteins to DNA, preserving their interactions. Chromatin Shearing: The DNA-protein complexes are fragmented into smaller pieces. Immunoprecipitation: Antibodies specific to the protein of interest are used to isolate the DNA-protein complexes. DNA Purification: The DNA is separated from proteins and other cellular material. Sequencing: The purified DNA is then sequenced to identify the binding sites of the proteins.
